![]() Choose Interpolation Calculator from the Analyze menu. If doing Beer’s law to determine the concentration of an unknown, place the unknown sample in the cuvette holder.Click Linear Fit,, to see the best fit line equation for the standard solutions.When finished, click to end data collection. Repeat Step 6 for the remaining samples.Enter the concentration of the second sample and click. Place your second sample in the cuvette slot.Enter the concentration of the sample and click. Place your first sample in the cuvette slot of the Spectrophotometer. Select boxes in the list or drag your cursor on the graph to select up to ten contiguous wavelengths. Option 3 If you wish to measure an average over a range of contiguous wavelengths of your choice, change Single 10 nm Band to Individual Wavelengths.You may then select up to ten wavelengths to measure at the same time. Option 2 If you wish to use the λ max chosen by Logger Pro, but you want the absorbance to be measured only at that one wavelength, change Single 10 nm Band to Individual Wavelengths.You can change the center wavelength value by clicking on the graph or by choosing a wavelength from the list. This measures the average absorbance from ~5 nm on either side of the chosen wavelength. Option 1 The default option is to use a single 10 nm band.There are three options when choosing a wavelength (or wavelengths) for subsequent measurements. The wavelength with the maximum value from the spectrum (λ max) will be automatically selected. Concentration as the data-collection mode. It becomes active when either the Concentration or Time mode is selected.Ĭonfigure Spectrometer data-collection dialog box List of wavelength options This column lists all the available wavelengths.You may wish to select a different wavelength. By default, the wavelength with the maximum measured value will be selected. Graph The graph displays a full-spectrum analysis of the sample in the cuvette holder.Concentration is selected, a wavelength or wavelengths will need to be chosen. If the measurement (Absorbance in this example) vs. Collection Mode The three options for data collection are offered.Click the Configure Spectrophotometer Data Collection button.Generate a spectrum as described above.To store the spectrum data, choose Store Latest Run from the Experiment menu.Place the sample in the Spectrophotometer and click. Fill a cuvette about 3/4 full of a sample of the solution to be tested.concentration for Beer’s law experiments, and absorbance (or %T) vs. wavelength, which produces a spectrum, absorbance (or %T) vs. There are three general types of data collection that measure absorbance or transmittance-absorbance (or %T) vs. Follow the instructions in the dialog box to complete the calibration, and then click.Align the cuvette so the clear side of the cuvette is facing the light source. After the Spectrophotometer has warmed up, place the blank cuvette in the Spectrophotometer. Fill a cuvette about 3/4 full with distilled water (or the solvent being used in the experiment) to serve as the blank. ![]() Note: For best results, allow the Spectrophotometer to warm up for a minimum of five minutes. ![]() To calibrate the SpectroVis Plus, choose Calibrate ► Spectrophotometer from the Experiment menu.Select the unit or data type you wish to measure.Ĭalibrate (Not Required if Measuring Intensity or Fluorescence).Choose Change Units ► Spectrophotometer from the Experiment menu.If you want to measure %T, fluorescence (excited at 405 nm or 500 nm), or intensity, do the following: If you want to measure the absorbance of a solution, proceed directly to the Calibrate section below. Select the Type of Data (or Units) You Want to Measure Connect the sensor following the steps in the Getting Started section of this user manual.
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